Published PCR primers targeting the ammonia monooxygenase gene (amoA) were applied to samples from activated sludge systems operated with low dissolved oxygen (DO) to quantify total and clade-level Nitrospira that perform complete ammonium oxidation (comammox); however. we found these existing primers resulted in significant artifact-associated non-target amplification. This not only ... https://theplayfulcollectivees.shop/product-category/toys/